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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore may add to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial job in advertising and marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two further splice variants have been noted but comprehensive nucleotide sequence information and facts is not but available.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide usually includes the epitope regarded by the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the best possible effects are...
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This guideline outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is certain.
Goal Info Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of each and every mitotic function. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is click here also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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